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Field
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experience with genotypic and phenotypic analyses • RT PCR and qPCR • scRNA-seq technologies • FACs analysis • Immunohistochemistry and tissue sectioning • Biochemical Assays
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advanced methods such as: a. quantitative PCR (qPCR), b. Western blotting, c. next-generation sequencing approaches (e.g., RNA-seq), d. flow cytometry. Experience working with mammalian cell cultures
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favourable. Experience in field studies and experimental design is desired. Experience with advanced molecular biology methods (e.g. [meta]genomics and/or [meta]transcriptomics), quantitative PCR, and
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. ● Experience with mouse models. ● Experience with cell and molecular biology techniques including PCR, western blotting, flow cytometry, cell culture and immunohistochemistry assays. Position Summary
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implementation of chromatographic separation techniques for metabolite profiling and quantitative PCR for gene expression analyses in gastrointestinal and other relevant tissues. By integrating this methodological
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of polysaccharides; 2. knowledge of polysaccharide analysis techniques; 3. knowledge of molecular cloning techniques and experience working with plants and plant tissue cultures; 4. experience in performing RT-PCR
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molecular biology techniques such as DNA extraction, PCR, or qPCR. Experience analyzing biological data using command line tools, R, SAS, or similar analytical software. Familiarity with bioinformatic
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). - Application of molecular and cellular biology techniques (PCR, Western blot, ELISA, immunofluorescence, and immunohistochemistry) in tumour samples and other tissues. - Characterization of the immune profile in
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University of North Carolina at Chapel Hill | Chapel Hill, North Carolina | United States | 1 day ago
+ color panels), tetramer staining, and intracellular cytokine detection Western blotting for phospho-proteins and signaling pathway analysis Proficiency in molecular biology techniques (qRT-PCR, Western
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extraction, cytokine ELISAs, quantitative RT-PCR, and basic epigenetic assays (e.g., histone-modification profiling). Prepare samples for transcriptomics (RNA-seq) and data interpretation. In vivo and BSL-2/3