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RAP opportunity at National Institute of Standards and Technology NIST Development of Technologies to Enable Single Molecule Protein Sequencing Location Material Measurement Laboratory
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RAP opportunity at National Institute of Standards and Technology NIST Design, Characterization, and Modeling of Sequence Controlled Polymers Location Material Measurement Laboratory, Materials
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that align DNA sequencing reads to the reference, but long and linked read sequencing technologies are now enabling haplotype-separated “de novo assembly” of human germline and cancer genomes. These methods
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, environmental, and microbiome analyses. Metagenomic sequencing holds the promise of enabling culture-free, non-targeted identification and characterization of a mixed population of bacteria, viruses, archaea, etc
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commercial assays. Common methods of characterization include copy number determination by quantitative PCR and digital PCR, as well as sequencing by Sanger and next-generation platforms. Projects focus
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benchmarks for genome sequencing methods [1]. In addition, these cell lines have been used as a well-characterized background DNA in over 50 commercial products. These cell lines, as well as induced
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of short tandem repeat (STR) markers by capillary electrophoresis and next-generation sequencing, mitochondrial genome sequencing, deconvolution of DNA mixtures using software and lab-based methods, rapid
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research focuses on detecting genomic variation that occurs during long-term culture and manufacturing stress. Projects may involve using Next-Generation Sequencing (NGS) to map transgene integration sites
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informatics developers in the public-private-academic Genome in a Bottle Consortium to develop methods to integrate short-, linked-, and long-read sequencing technologies to form benchmarks for somatic variant
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distinct species or strains, and currently only molecular methods (e.g. polymerase chain reaction, sequencing) are able to differentially enumerate microbes in such a mixture. However, measurement bias in