Long non-coding RNAs (lncRNAs) in microenvironmental interactions of B cell chronic lymphocytic leukemia

Organisation/Company

CEITEC MU

Research Field

Biological sciences
Biological sciences » Biology
Chemistry » Biochemistry

Researcher Profile

First Stage Researcher (R1)

Country

Czech Republic

Application Deadline

15 Feb 2026 - 22:50 (UTC)

Type of Contract

Temporary

Job Status

Full-time

Offer Starting Date

1 Jul 2025

Is the job funded through the EU Research Framework Programme?

Not funded by a EU programme

Is the Job related to staff position within a Research Infrastructure?

No

Doctoral study program: Biomedical Sciences
Specialization: Molecular Medicine

Supervisor: Prof. Marek Mraz, MSc., M.D., Ph.D.

Topic title: Long non-coding RNAs (lncRNAs) in microenvironmental interactions of B cell chronic lymphocytic leukemia

Annotation:

Marek Mraz research group has a long-term interest in non-coding RNAs and microenvironmental interactions of malignant B cells, and this research has been supported by an ERC Starting grant (2019-2024). We have previously described novel regulators of microenvironmental interactions including short non-coding RNAs, microRNAs (Sharma et al…Mraz, Blood, 2021; Musilova et al…Mraz, Blood, 2018; Cerna et al…Mraz, Leukemia, 2019). MicroRNAs were shown to play a pivotal role in B cell functions; however, the functions of long non-coding RNAs (lncRNAs) remain unclear. We aim to decipher for the first time the role of lncRNAs in B cell receptor (BCR) signaling and B-T cell interactions. Human genome contains large numbers of lncRNAs that can regulate various physiological cellular processes or contribute to the onset or aggressiveness of cancer. We will study lncRNAs in the context of chronic lymphocytic leukemia (CLL), which is driven by aberrations in the BCR pathway and B-T interactions. Regulation of BCR pathway and B-T cell interactions by lncRNAs is likely of relevance for CLL, but is also transferable to the biology of other B cell malignancies, autoimmune diseases and normal B cells. We identified 3 candidate lncRNAs involved in microenvironmental interactions of CLL. We will decipher the molecular functions of these lncRNAs using biochemical and cellular approaches and via a novel lncRNA knock-out mouse model. We have engineered mice for genetic loss of one of these lncRNAs, and the student will analyse the phenotype of these mice and breed them with known CLL mouse models (Eu-TCL1). Detailed biochemical/molecular studies will complement these data and we will also analyze primary samples from patients with B cell malignancies. We will identify functions of lncRNAs using CRISPR interference, RNA pulldown experiments, mouse models, and molecular biology technics. Furthermore, we developed a novel co-culture model inducing robust primary CLL cell proliferation (~50%) in vitro (Hoferkova et al, Leukemia, 2024). We aim to utilize this game-changing tool to perform the first-ever CRISPR screening of lncRNAs/genes regulating primary CLL cell proliferation. This will help better understand the disease biology and possibly identify novel molecular targets for therapy.

Recommended literature:

• Zeni and Mraz LncRNAs in adaptive immunity: role in physiological and pathological conditions.  RNA Biol. 2021 May;18(5):619-632. https://pubmed.ncbi.nlm.nih.gov/33094664
• Mattick JS, et al. Long non-coding RNAs: definitions, functions, challenges and recommendations. Nat Rev Mol Cell Biol. 2023 Jun https://pubmed.ncbi.nlm.nih.gov/36596869/

• Sharma et al. …Mraz. miR-29 Modulates CD40 Signaling in Chronic Lymphocytic Leukemia by Targeting TRAF4: an Axis Affected by BCR inhibitors. Blood 2021. https://pubmed.ncbi.nlm.nih.gov/33171493/
• Musilova K, Mraz M. MicroRNAs in B-cell lymphomas: how a complex biology gets more complex. Leukemia. 2015 May;29(5):1004-17

Research area: Cancer biology

Keywords: lymphoma, CLL, lncRNA, microenvironment

Funding for the PhD candidate:

In the academic year 2026/27 the net income of the CEITEC PhD School student is expected to be at least CZK 29,400 (approx. EUR 1215)
Part-time salary (min. 0,5 FTE) on EHA grant/AZV/GACR grants + doctoral scholarship + CEITEC PhD School scholarship.

Requirements on candidates:

• Motivated smart people that have the “drive” to work independently, but also willing to learn from other people in the lab and collaborate.
• Candidates should have a master’s degree in Molecular biology, Biochemistry, or similar field and have deep interest in molecular biology and cancer cell biology.

Information about the supervisor: H-index 30 (citations > 3500, 50 publications with IF), currently principal investigator of 4 grants (AZV 3x, NPO, in the past ERC Starting grant). Dr. Mraz has currently 7 PhD students, with 3 finishing soon). international collaborations: University of Southampton, Univ.California- San Diego, Mayo Clinic, Dana-Farber Cancer Institute, EMBL, University of Turin (student internship available), member of EHA Comittee, reviewer in scientific journals: Blood, Leukemia, Leukemia Research; https://is.muni.cz/auth/osoba/101627 ;
More information about the research group: http://mrazlab.ceitec.cz/

Start date: September 2026

Sign-up link:https://www.ceitec.eu/long-non-coding-rnas-lncrnas-in-microenvironmental-interactions-of-b-cell-chronic-lymphocytic-leukemia/t11430

Website

https://www.ceitec.eu/long-non-coding-rnas-lncrnas-in-microenvironmental-intera…

Number of offers available

1

Company/Institute

CEITEC MU

Country

Czech Republic

City

Brno

Postal Code

625 00

Street

Kamenice 753/5

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