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warming (EvoLatitude)”. The GENERAL OBJECTIVE of EvoLatitude is to elucidate the mechanisms of the evolution of latitudinal ranges by exploring how traits and genetic composition in seven related Gomphidae
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of biotechnological interest, mainly Saccharomyces cerevisiae during winemaking. We are focused in regulatory systems as stress response (mainly oxidative) and nutrient signaling pathways, and we use genetic
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signaling pathways, and we use genetic manipulation to characterize such mechanisms in conditions that mimic industrial environments. The Computational Synthetic Biology group (CSBG), led by Dr. Irene Otero
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. Cell culture and genetic manipulation, including lentivirus production, transduction, transfection, and CRISPR/Cas9 genome editing. 2. Live-cell imaging using confocal microscopy, along with quantitative