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of the Principal Investigator, isolates extracellular vesicles (EVs) from human blood samples to quantify EV concentration and examine cargo material using techniques such as RNA sequencing (RNAseq), Fluorescence
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, primary neuronal and glial cell culture. Must have experience maintaining a mouse colony, as well as stereotactic surgery, brain dissection, immunofluorescence, fluorescence microscopy, and confocal
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to laboratory standard operating procedures. Essential Duties and Responsibilities include the following: Performs standard experiments in the lab: Microscopy (light, fluorescent, or confocal); electrophoresis
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, fluorescent, or confocal); electrophoresis (nucleic acid and protein); PCR; Western blotting; flow cytometry (multiparameter and spectral) of mouse and human specimens; DNA/RNA extraction; preparation
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-generation sequencing, in situ single-cell analyses of DNA and RNA by Fluorescent in situ Hybridization (FISH), and CRISPR gene editing to induce genomic instability and study its consequences on tumor