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immunofluorescence, in situ hybridization, genotyping, quantitative PCR, micro-computed tomography, fluorescent and confocal microscopy imaging, cell culture, and computer based data analysis. Digital PCR and new
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proteins, we will employ a genetic code expansion strategy, developed in the group, to incorporate small reactive non-canonical amino acids. This allows us to attach fluorescent probes at the desired
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radiation beamline hands-on experience in X?ray microscopy techniques, X?ray diffraction or X?ray fluorescence working knowledge of image processing e.g. using machine learning German skills For further
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of the Principal Investigator, isolates extracellular vesicles (EVs) from human blood samples to quantify EV concentration and examine cargo material using techniques such as RNA sequencing (RNAseq), Fluorescence
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spectroscopic techniques [Two-Dimensional Electronic Spectroscopy (2DES), Transient Absorption, and Transient Fluorescence (with a Streak camera)]. To run, maintain in good condition and optimize when needed
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protected and open field cultivation systems Use lab and field equipment (ICP, HPLC, gas exchange analyzer, soil/root/plant sensors, chlorophyll fluorescence, etc.), data loggers and image processing tools
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staining, Golgi staining, and use of fluorescent probes to interrogate cellular and subcellular mechanisms. Apply advanced subcellular fractionation workflows to isolate mitochondria, ER, MAM, plasma
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. Key responsabilities will include the synthesis and validation of dual modal nanomaterial for Image guided surgery applications. PET will be used for locating and staging tumours, fluorescence
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, primary neuronal and glial cell culture. Must have experience maintaining a mouse colony, as well as stereotactic surgery, brain dissection, immunofluorescence, fluorescence microscopy, and confocal
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DNA and RNA extraction and quantification, Western blotting, Real-time, Immunoprecipitation and ELISA Experience with immunofluorescence and fluorescence microscopy Excellent interpersonal skills and