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Field
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will be responsible for establishing and maintaining colonies of genetically modified mice, including fluorescence reporter lines and mice with mutations causing JE defects. In addition, the technician
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sensitive fluorescent probes to identify, localise and quantitatively assess the activity of selected proteases in cancer cells, neutrophils, PBMCs and tissue samples. The use of advanced confocal microscopy
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ongoing developments deploying the escape-time technology on a broad range of measurement problems in biology. This position requires expertise in single molecule fluorescence experiments: specifically
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, fluorescence lifetime and vibrational imaging methods. Large-scale and high-throughput imaging and analysis pipelines: data acquisition and analysis to characterize cell-types and connectivity in mammalian brain
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crosslinks and how this pathway contributes to bacterial survival following antibiotic treatment. This work will be tightly coordinated with live-cell fluorescent microscopy approach undertaken in parallel in
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the condensed phase (e.g., optical cavity-based direct absorption, fluorescence, confocal microscopy, time correlated single photon counting, etc.). Familiarity with magnetic field effects in proteins and/or
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rats; 3) Molecular techniques: ELISA, Western blot, immunofluorescence and fluorescence/confocal microscopy; 4) Brain dissection of young and adult rats; 5) Statistical analysis. Desirable requirements
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conjugation for fluorescence dye labeling Proficient in data analysis and presentation Excellent communication skills (oral and written) along with a strong publication record. Additional Information
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form (fluorescence or CD or NMR or EPR, for example). Required License/Registration/Certification N/A Physical Requirements Active work at lab bench, move between lab rooms, push carts and carry flasks
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will apply include endogenous protein tagging by CRISPR and integrases, in-cell interaction proteomics by proximity labeling, subcellular localization by spatial proteomics and fluorescence microscopy