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in vivo murine studies, including models of: Burn injury, alcohol, trauma and colitis Perform molecular and cellular biology techniques such as: Western blotting, ELISA RNA/DNA isolation, RT-qPCR
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). - Application of molecular and cellular biology techniques (PCR, Western blot, ELISA, immunofluorescence, and immunohistochemistry) in tumour samples and other tissues. - Characterization of the immune profile in
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University of North Carolina at Chapel Hill | Chapel Hill, North Carolina | United States | 2 days ago
, cytokine production analysis ELISA and multiplex cytokine quantification Tissue explant culture and ex vivo functional assessments Advanced flow cytometry skills including multi-parameter analysis (up to 15
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advanced biochemical methods (e.g., SPR, BLI, ELISA, fluorescence-based assays) Has experience with yeast display Has strong documentation of peer-reviewed publications Demonstrates the ability to work
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cellular immune responses (ELISA). Contribute to the analysis and interpretation of experimental data generated within the project. Participate in project meetings and other activities necessary
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extraction, cytokine ELISAs, quantitative RT-PCR, and basic epigenetic assays (e.g., histone-modification profiling). Prepare samples for transcriptomics (RNA-seq) and data interpretation. In vivo and BSL-2/3
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perform cellular and molecular experiments (e.g. flow cytometry, ELISA/Luminex, qPCR, Western blot, cell culture, primary immune cell work), and analyse/interrogate datasets together with the team. Support
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techniques, including DNA/RNA extraction, RT-PCR, flow cytometry, ELISA, Western blotting, protein purification, and cell-based cytotoxicity assays. Hands-on experience with small animal models, including
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samples as well as mouse models. The candidate is expected to be able to perform animal tissue harvest and processing, flow cytometry, FACS sorting, cell culture, nucleic acid extractions, ELISAs, bead
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University of North Carolina at Chapel Hill | Chapel Hill, North Carolina | United States | 2 days ago
, immunoblotting, ELISA), and data analysis. Experience with high-throughput transcriptional profiling techniques (e.g., RNA-seq), bioinformatics, and large-scale data analysis is desirable. The ideal candidate will