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Field
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on isolated mutations in patients with telomeric disorders Construction of KI cell lines using CRISPR-Cas9 Characterization of the telomeric phenotypes of these KI lines Characterization of the phenotypes
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DNA from soils Carry out library preparation for long read sequencing Use CRISPR-Cas to edit genomes of common soil bacteria Establish and maintain cultures of common freshwater Cyanobacteria This role
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high-resolution confocal and live-cell imaging approaches. Working with human and mammalian cell lines, you will investigate virus–host interactions using molecular biology, virology, CRISPR/siRNA-based
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models. In addition, it will use Crispr-Cas9 approach to knockdown important transcription factors, such as Snail and Twist, in triple-negative breast cancer cell lines to examine the functional role and
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University of North Carolina at Chapel Hill | Chapel Hill, North Carolina | United States | about 12 hours ago
follow-up experiments using CRISPR-based techniques applied to primary human cells grown in an ex vivo system. Hundreds of data sets have already been collected and are ready to be analyzed. Prior
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integrates CRISPR-based genome engineering, quantitative and live-cell microscopy, biochemistry, and computational analysis to dissect how cells sense and respond to replication-associated threats. Recent work
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perturbation strategies, including CRISPR-based gene editing and pharmacological inhibition, to identify mechanistic vulnerabilities in disease-relevant contexts. The candidate will contribute to experimental
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in addition to established cell lines. HIV laboratory research or related virology. Mouse models, including tissue harvesting and in vivo experimental work. CRISPR-based genetic perturbation approaches
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research project The project will address distinct metabolic pathways in prostate cancer progression. The study is based on an in vivo CRISPR mouse model for prostate cancer, introducing multiple mutations
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mechanism of CRISPR-associated nucleotide signaling. We also aim to develop innovative tools for fast and sensitive nucleic acid detection and the diagnosis of human disease, using insights gained from our