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Field
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assess candidate functions using CRISPR and transgenic approaches. Anticipated Division of Time Research (55%): The Postdoctoral Associate will play a leading role in conducting experimental, computational
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, transport, and resistance, and potentially assess candidate functions using CRISPR and transgenic approaches. Anticipated Division of Time Research (55%): The Postdoctoral Associate will play a leading role
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practical experience of state-of-the-art molecular biology techniques and data analysis. In particular expertise in the following is essential : Genome editing, Epigenome editing, Off-target analysis, CRISPR
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and non-myocyte cells; utilizes CRISPR-Cas9 genome editing to model genetic cardiovascular diseases; performs molecular biology techniques (qPCR, Western blotting, cloning, library preparation for omics
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(e.g. RNAi, CRISPR/Cas9, small-molecules). In this context, we also develop new computational tools for automated analysis and data visualization. These include algorithms and software applications
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expertise, we are creating functional genetic maps using conditional CRISPR/Cas9-based single and higher-order knockout perturbations combined with single-cell expression profiling and imaging. We expect
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at the interface of microbiology and functional genomics, and more specifically develop transposon- and CRISPR-based approaches to characterize genome-wide regulation of bacterial defence systems
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. MATLAB programming ability. Strong skillset/background in biochemical and biophysical methods and approaches, standard molecular biology and protein chemistry techniques, CRISPR/Cas, and/or computational
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progression and therapy resistance. This position will leverage cutting-edge approaches including spatial transcriptomics, CRISPR-based functional genomic screening, and patient-derived xenograft (PDX) models
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or iPSCs). · Strong experimental skills in molecular and cellular biology, including techniques such as RNA/protein expression analysis, CRISPR/Cas9 gene editing, immunofluorescence, flow cytometry, and