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Field
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culture. Extensive expertise in mammalian cell genetic manipulation (CRISPR/Cas9, RNAi, gene complementation, lentiviral transduction). Extensive expertise in molecular cloning, protein purification and
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will: Develop and utilize human iPSC-derived cardiomyocyte models. Apply genome-editing technologies, including CRISPR-based methods. Perform mechanistic studies integrating electrophysiology and
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/bayesian/deep-learning analyses, with functional validation in spruce via CRISPR-Cas9 and nanoparticle delivery. The postdoc will join Professor Nathaniel R. Street’s team at UPSC, working closely with
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., Western blot, PCR, transfection, construct design, shRNA/CRISPR, immunostaining, fluorescence microscopy, live imaging) Preferred expertise in one or more of the following areas: cancer biology, vascular
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genomics approaches. Familiarity with computational analysis (e.g., Python, R, Bash) and/or genome-scale data integration. Experience with cell culture and functional genomics (e.g., CRISPR/Cas9), cancer
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, genetic engineering with CRISPR, disease modeling, transplantation into animal models of diabetes, biomedical engineering, bioinformatics, and single-cell sequencing technologies. Job Description Primary
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of single cell and next-generation RNA sequencing, and proteomic analysis of kinome data. Advanced molecular biology techniques including gene editing (CRISPR), plasmid design and production, bacterial
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-bacterial/microbial-host interactions using molecular, cellular, and tissue-level assays. Designs, performs, and analyzes experiments involving gene perturbation, gene editing (CRISPR/Cas9, RNAi), and gene
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. Likewise, experience with retro- and lenti-viral production and transduction, RNAi-based strategies for post-transcriptional regulation of gene expression and CRISPR/Cas9 techniques are desired. Additionally
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or equivalent Skills/Qualifications Communication, Organisational skills, Research skills, Crispr Cas9 to E. coli, Python, R, Java, C, C++, Statistical analysis, Experience in Biosensors, Experience in 3D