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Field
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, transport, and resistance, and potentially assess candidate functions using CRISPR and transgenic approaches. Anticipated Division of Time Research (55%): The Postdoctoral Associate will play a leading role
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-fluorescence microscopy, flow cytometry and immunoblotting. CrispR cas9 is routinely used to create mammalian cell knockouts. RNAseq and proteomics-based approaches are also anticipated. Work will involve
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or iPSCs). · Strong experimental skills in molecular and cellular biology, including techniques such as RNA/protein expression analysis, CRISPR/Cas9 gene editing, immunofluorescence, flow cytometry, and
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immune activation, cytotoxicity, and persistence. Additional methods may involve CRISPR-based perturbations, viral vector engineering, and immune cell manufacturing workflows relevant to CAR-based
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. MATLAB programming ability. Strong skillset/background in biochemical and biophysical methods and approaches, standard molecular biology and protein chemistry techniques, CRISPR/Cas, and/or computational
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DNA from soils Carry out library preparation for long read sequencing Use CRISPR-Cas to edit genomes of common soil bacteria Establish and maintain cultures of common freshwater Cyanobacteria This role
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high-resolution confocal and live-cell imaging approaches. Working with human and mammalian cell lines, you will investigate virus–host interactions using molecular biology, virology, CRISPR/siRNA-based
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elements and regulatory factors control gene expression in both physiological and disease contexts. Current projects leverage ReLiC, a high-throughput CRISPR screening platform that we recently developed
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integrates CRISPR-based genome engineering, quantitative and live-cell microscopy, biochemistry, and computational analysis to dissect how cells sense and respond to replication-associated threats. Recent work
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models. In addition, it will use Crispr-Cas9 approach to knockdown important transcription factors, such as Snail and Twist, in triple-negative breast cancer cell lines to examine the functional role and