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Field
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., Western blot, PCR, transfection, construct design, shRNA/CRISPR, immunostaining, fluorescence microscopy, live imaging) Preferred expertise in one or more of the following areas: cancer biology, vascular
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-bacterial/microbial-host interactions using molecular, cellular, and tissue-level assays. Designs, performs, and analyzes experiments involving gene perturbation, gene editing (CRISPR/Cas9, RNAi), and gene
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of single cell and next-generation RNA sequencing, and proteomic analysis of kinome data. Advanced molecular biology techniques including gene editing (CRISPR), plasmid design and production, bacterial
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. Likewise, experience with retro- and lenti-viral production and transduction, RNAi-based strategies for post-transcriptional regulation of gene expression and CRISPR/Cas9 techniques are desired. Additionally
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-leading research in several fields. Notably, the groundbreaking discovery of the CRISPR-Cas9 gene-editing tool, which was awarded the Nobel Prize in Chemistry, was made here. At Umeå University, everything
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and/or MD (or equivalent) degree in hand by start date. Preferred applicants will have a background in molecular biology (e.g., CRISPR/Cas9, lentivirus, adenovirus), lipid signaling, lipidomics, or RNA
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engineering (e.g. CRISPR/Cas or related techniques - essential) Prior experience with epigenetic editing or methods will be advantageous (but is not required for an applicant to be considered) Experience in
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) and their relationships with neighboring tissues in pathophysiological conditions. We utilize in vivo mouse genetics, live imaging, 3D organoids, genome-wide Cas9/Crispr based functional genetic
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manufacturing Use and further develop CRISPR (epi-)genome editing methods in CAR T cells Using cellular (e.g., FACS) and molecular (e.g., single cell analysis, next generation sequencing) methods to characterize
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-leading research in several fields. Notably, the groundbreaking discovery of the CRISPR-Cas9 gene-editing tool, which was awarded the Nobel Prize in Chemistry, was made here. At Umeå University, everything