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models. In addition, it will use Crispr-Cas9 approach to knockdown important transcription factors, such as Snail and Twist, in triple-negative breast cancer cell lines to examine the functional role and
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mechanism of CRISPR-associated nucleotide signaling. We also aim to develop innovative tools for fast and sensitive nucleic acid detection and the diagnosis of human disease, using insights gained from our
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research project The project will address distinct metabolic pathways in prostate cancer progression. The study is based on an in vivo CRISPR mouse model for prostate cancer, introducing multiple mutations
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University of North Carolina at Chapel Hill | Chapel Hill, North Carolina | United States | 3 days ago
follow-up experiments using CRISPR-based techniques applied to primary human cells grown in an ex vivo system. Hundreds of data sets have already been collected and are ready to be analyzed. Prior
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in addition to established cell lines. HIV laboratory research or related virology. Mouse models, including tissue harvesting and in vivo experimental work. CRISPR-based genetic perturbation approaches
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culture. Extensive expertise in mammalian cell genetic manipulation (CRISPR/Cas9, RNAi, gene complementation, lentiviral transduction). Extensive expertise in molecular cloning, protein purification and
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/bayesian/deep-learning analyses, with functional validation in spruce via CRISPR-Cas9 and nanoparticle delivery. The postdoc will join Professor Nathaniel R. Street’s team at UPSC, working closely with
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will: Develop and utilize human iPSC-derived cardiomyocyte models. Apply genome-editing technologies, including CRISPR-based methods. Perform mechanistic studies integrating electrophysiology and
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-biochemistry (SPR, polarization) and molecular cell biology (Gateway cloning, Gibson assembly, CRISPR/Cas9 etc). Tasks: Conduct self-driven experimental research towards the lab aims Opportunity to contribute
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genomics approaches. Familiarity with computational analysis (e.g., Python, R, Bash) and/or genome-scale data integration. Experience with cell culture and functional genomics (e.g., CRISPR/Cas9), cancer