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screening. The researcher will prepare DUS–tRNA complexes for structural analysis using single-particle cryo-EM. He/she will perform sample screening and optimization for cryo-EM data collection and analyze
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of thousands of particle projections. As a result, flexible and dynamic regions—such as glycans or protein loops—are often blurred during processing and remain unresolved in the final maps. However, traces of
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Bank (EMDB) for Glycan Awareness.” In cryo-electron microscopy (cryo-EM), structural models are generated by averaging tens of thousands of particle projections. Flexible and dynamic regions—such as
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