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applied chemical matrix (matrix assisted laser desorption/ionization or MALDI). However, MALDI-MSI can also be performed using infrared (IR) lasers. IR-MALDI offers several advantages over UV-MALDI, such as
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. However, this typically results in a larger tissue with a disorganized matrix architecture, not resembling native cartilage. To unlock the full potential of microtissue-based TE, a deeper understanding is
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and dissolution of the solid matrix. These pore space adaptions are often strongly and non-linearly coupled to the relevant transport phenomena, significantly influencing the overall performance and
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(embryonic) tissues. These techniques enable non-invasive, bio-friendly manipulation of cells and their surrounding matrix, allowing investigation of how physical forces and ECM properties drive tissue