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group aims to determine regulatory pathways affected by disease by implementing the use of spatial proteomics combined with transcriptomics and live imaging. The total proteome of a neuron includes a vast
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experience of the laboratory delivery of multiplex imaging datasets of tissue samples as well as significant laboratory experience of general methods including immunoassays and cell culture. You will have
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to target specific transcription factors (iii) use of high content imaging and AI to phenotype these cultures (iii) use of bulk and single-cell RNAseq to characterise the transcriptional profile of each cell
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techniques, multispectral flow cytometry, and bioinformatic analysis of transcriptomic (RNAseq) datasets. Experience with image analysis, particularly for immunofluorescence, is desirable. You should be
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the Medical Research Council and Medical and Life Sciences Translational Fund. It offers an exciting opportunity to work in a vibrant multi-disciplinary team that crosses the domains of clinical imaging and
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participation, working with PPI, to choose a creative ‘menu’ of participation modes, e.g., a digital pinboard for images and memes and recruit parents, children, and clinicians to participate in qualitative
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cilia function. This exiting project will combine our recently developed centrosome purification method with advanced proteomics and high-resolution imaging to deepen our understanding of these processes