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Institute of Experimental Botany, The Academy of Sciences of the Czech Republic | Czech | about 2 months ago
-ground tissues. Methodologically, the project will involve advanced live cell imaging approaches, benefiting from the specialized microscopy available in the IEB. Specifically, the focus will be
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morphogenesis, epithelial differentiation, and hormonal responsiveness. Using advanced imaging methodologies such as confocal microscopy and live-cell imaging, the structural organization and dynamic behavior
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models, the student will explore how DNA replication and transcription and DNA damage response regulate differentiation of luminal cells to milk-secreting alveolar cells. Advanced imaging techniques
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kleptoplastidic dinoflagellates along a range of plastid integration levels. You will be involved in manual and automated image-based isolation, culture establishment, (single-cell) transcriptomic data generation
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, the only one in the country housed at the Institute of Biophysics of the CAS. This advanced technology integrates spectral flow cytometry with sorting and image analysis, enabling precise, high-dimensional
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integration levels. You will be involved in manual and automated image-based isolation, culture establishment, (single-cell) transcriptomic data generation and determination of physiological parameters of known
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: a general wet-lab, two labs for bacterial and eukaryotic cultivation, and two for molecular biology. Instrumentation: A fully automated fluorescence microscope with image analysis for high-throughput
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. These tools will be employed to study the signalling dynamics in cell cultures and organoid models using advanced live-cell imaging techniques. We will employ transcriptomic and proteomic analyses in healthy
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by atmospheric-chemistry modelling, with emphasis on regional and local (urban) scales. Another goal of the subject is to improve more detailed picture of the feedbacks of methane in the climate system