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on mouse models of retinal ganglion cell neurodegeneration, in vivo microscopy, AAV mediated gene delivery and knockout, along with transcriptomic and immunohistological analyses. The postdoctoral researcher
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. Computational and bioinformatic skills. Experience in microscopy. Generation and analysis of mouse models. Handling of human samples. Molecular biology skills including CRISPR, cloning and qPCR. In vitro cell
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healing, and nuclear mechanosensing. The successful candidate will have established experience in the following areas: (a) imaging techniques such as fluorescence, confocal, and multi-photon microscopy, (b
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delivery (e.g., mRNA, siRNA, ASO, lentivirus technology, transient transfection, loss/gain of function experiments), immunocytochemistry, light and confocal microscopy. Prior experience with induced
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. Techniques include flow cytometry, immune cell transfers, cloning and molecular biology, biochemistry, cell/tissue culture, fluorescence and confocal microscopy, and analysis of RNAseq or other data sets